Phospho-Axl (Y702) Recombinant Rabbit Monoclonal Antibody [PSH17-98]
cat.: HA723963
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH17-98 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 98 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Tyr702 of human Axl. |
| Positive control: | NCI-H1299 cell lysate, NCI-H1299 treated with 100ng/mL Gas6 for 10 minutes cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P30530 Human |
| Alternative names: | Adhesion related kinase AI323647 Ark Axl AXL oncogene AXL receptor tyrosine kinase AXL transforming gene AXL transforming sequence/gene EC 2.7.10.1 JTK11 Oncogene AXL Tyro7 Tyrosine protein kinase receptor UFO Tyrosine-protein kinase receptor UFO UFO UFO_HUMAN |
Images
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Fig1:
Western blot analysis of Phospho-Axl (Y702) on different lysates with Rabbit anti-Phospho-Axl (Y702) antibody (HA723963) at 1/5,000 dilution. Lane 1: NCI-H1299 cell lysate Lane 2: NCI-H1299 treated with 100ng/mL Gas6 for 10 minutes cell lysate Lane 3: NCI-H1299 treated with 100ng/mL Gas6 for 10 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 98 kDa Observed band size: 140 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723963) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.