5 Lipoxygenase Recombinant Rabbit Monoclonal Antibody [PSH18-05]
cat.: HA723968
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH18-05 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 78 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human 5 Lipoxygenase aa 151-200. |
| Positive control: | HL-60 cell lysate, K-562 cell lysate, Rat placenta tissue lysate, Daudi cell lysate, Mouse placenta tissue lysate. |
| Subcellular location: | Cytoplasm, Nucleus matrix, Nucleus membrane, Cytoplasm, perinuclear region, cytosol, Nucleus envelope, Nucleus intermembrane space. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P09917 Human | P48999 Mouse | P12527 Rat |
| Alternative names: | 5 Lipoxygenase 5 LO 5 LOX 5-lipoxygenase 5-LO 5-LOX 5LOX 5LPG ALOX 5 Alox5 Arachidonate 5 lipoxygenase Arachidonate 5-lipoxygenase arachidonic 5-lipoxygenase alpha-10 isoform arachidonic 5-lipoxygenase delta-10-13 isoform arachidonic 5-lipoxygenase delta-13 isoform arachidonic 5-lipoxygenase delta-p10 isoform Arachidonic acid 5 lipoxygenase Leukotriene A4 synthase LOG 5 LOG5 LOX5_HUMAN MGC163204 |
Images
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Fig1:
Western blot analysis of 5 Lipoxygenase on different lysates with Rabbit anti-5 Lipoxygenase antibody (HA723968) at 1/5,000 dilution. Lane 1: HL-60 cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: Rat placenta tissue lysate (30 µg/Lane) Predicted band size: 78 kDa Observed band size: 75 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723968) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of 5 Lipoxygenase on different lysates with Rabbit anti-5 Lipoxygenase antibody (HA723968) at 1/5,000 dilution. Lane 1: Daudi cell lysate (20 µg/Lane) Lane 2: Mouse placenta tissue lysate (30 µg/Lane) Predicted band size: 78 kDa Observed band size: 75 kDa Exposure time: 1 minute 50 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723968) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.