PADI2 Recombinant Rabbit Monoclonal Antibody [PSH18-06]
cat.: HA723969
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH18-06 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 76 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PADI2 aa 1-350. |
| Positive control: | PANC-1 cell lysate, SK-Br-3 cell lysate, MCF7 cell lysate, HT-29 cell lysate, human breast cancer tissue, human colon tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:200-1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q9Y2J8 Human |
| Alternative names: | KIAA0994 OTTHUMP00000044625 PAD 2 PAD H19 PAD-H19 PAD2 PADI 2 Padi2 PADI2 protein PADI2_HUMAN PDI 2 PDI2 Peptidlyarginine deiminase type II Peptidyl arginine deiminase II Peptidyl arginine deiminase type II Peptidylarginine deiminase II Protein arginine deiminase Protein arginine deiminase type 2 Protein arginine deiminase type II Protein-arginine deiminase type II Protein-arginine deiminase type-2 |
Images
|
Fig1:
Western blot analysis of PADI2 on different lysates with Rabbit anti-PADI2 antibody (HA723969) at 1/5,000 dilution. Lane 1: PANC-1 cell lysate (20 µg/Lane) Lane 2: SK-Br-3 cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (low expression) (20 µg/Lane) Lane 4: HT-29 cell lysate (20 µg/Lane) Predicted band size: 76 kDa Observed band size: 75 kDa Exposure time: Lane 1-2: 6 seconds; Lane 3-5: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723969) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-PADI2 antibody (HA723969) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723969) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PADI2 antibody (HA723969) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723969) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
PADI2 was immunoprecipitated from 0.2 mg PANC-1 cell lysate with HA723969 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723969 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: PANC-1 cell lysate (input) Lane 2: HA723969 IP in PANC-1 cell lysate Lane 3: Rabbit IgG instead of HA723969 in PANC-1 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 2 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.