HSD3B1 Recombinant Rabbit Monoclonal Antibody [PSH18-08]
cat.: HA723971
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH18-08 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human HSD3B1 aa 1-300. |
| Positive control: | Caco-2 cell lysate, Mouse ovary tissue lysate, Rat ovary tissue lysate, mouse adrenal gland tissue, mouse ovary tissue, rat adrenal gland tissue, rat ovary tissue. |
| Subcellular location: | Endoplasmic reticulum membrane, Mitochondrion membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200 |
| Uniprot #: | SwissProt: P14060 Human | P24815 Mouse | P22071 Rat |
| Alternative names: | 3 beta and steroid delta isomerase 1 3 beta and steroid hydroxy delta 5 steroid dehydrogenase 3 beta HSD I 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I 3-beta-HSD I 3-beta-hydroxy-5-ene steroid dehydrogenase 3BETAHSD 3BH 3BHS1_HUMAN 3BHSD Delta-5-3-ketosteroid isomerase HGNC:5217 HSD3B HSD3B1 HSDB3 HSDB3A Hydroxy delta 5 steroid dehydrogenase 3 beta and steroid delta isomerase 1 Hydroxy delta 5 steroid dehydrogenase Progesterone reductase SDR11E1 Steroid Delta-isomerase trophoblast antigen Trophoblast antigen FDO161G |
Images
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Fig1:
Western blot analysis of HSD3B1 on different lysates with Rabbit anti-HSD3B1 antibody (HA723971) at 1/5,000 dilution. Lane 1: Caco-2 cell lysate (20 µg/Lane) Lane 2: Caco-2 cell lysate (no heat) (20 µg/Lane) Lane 3: Mouse ovary tissue lysate (20 µg/Lane) Lane 4: Rat ovary tissue lysate (20 µg/Lane) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: Lane 1-2: 20 seconds; Lane 3-4: 1 minute 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723971) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue with Rabbit anti-HSD3B1 antibody (HA723971) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723971) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-HSD3B1 antibody (HA723971) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723971) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue with Rabbit anti-HSD3B1 antibody (HA723971) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723971) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-HSD3B1 antibody (HA723971) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723971) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.