MURF1 Recombinant Rabbit Monoclonal Antibody [PSH18-10]
cat.: HA723973
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, FC, IP
Clonality: Monoclonal
Clone number: PSH18-10
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 40 kDa
Isotype: IgG
Immunogen: Recombinant protein within human MURF1 aa 180-343 /353
Positive control: SK-MEL-28 cell lysate, Mouse skeletal muscle tissue lysate, Mouse heart tissue lysate, Rat skeletal muscle tissue lysate, Rat heart tissue lysate, SK-MEL-28.
Subcellular location: Cytoplasm, Nucleus, myofibril, sarcomere, M line, Z line.
Recommended Dilutions:
  WB
  FC
  IP
1:5,000
1:1,000
1-2μg/sample
Uniprot #: SwissProt: Q969Q1 Human | Q38HM4 Mouse | Q91Z63 Rat
Alternative names: IRF MURF1 RNF28 SMRZ TRIM63 E3 ubiquitin-protein ligase TRIM63 Iris RING finger protein Muscle-specific RING finger protein 1 RING finger protein 28 RING-type E3 ubiquitin transferase TRIM63 Striated muscle RING zinc finger protein Tripartite motif-containing protein 63 MuRF-1 MuRF1
Images
HA723973_1.jpg Fig1: Western blot analysis of MURF1 on different lysates with Rabbit anti-MURF1 antibody (HA723973) at 1/5,000 dilution.

Lane 1: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 2: Mouse skeletal muscle tissue lysate (40 µg/Lane)
Lane 3: Mouse liver tissue lysate (negative) (40 µg/Lane)
Lane 4: Mouse heart tissue lysate (40 µg/Lane)

Predicted band size: 40 kDa
Observed band size: 40 kDa

Exposure time:1 minute 3 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723973) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA723973_2.jpg Fig2: Western blot analysis of MURF1 on different lysates with Rabbit anti-MURF1 antibody (HA723973) at 1/5,000 dilution.

Lane 1: Rat skeletal muscle tissue lysate (40 µg/Lane)
Lane 2: Rat liver tissue lysate (negative) (40 µg/Lane)
Lane 3: Rat heart tissue lysate (40 µg/Lane)

Predicted band size: 40 kDa
Observed band size: 40 kDa

Exposure time: 59 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723973) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA723973_3.jpg Fig3: Flow cytometric analysis of SK-MEL-28 cells labeling MURF1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA723973, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA723973_4.jpg Fig4: MURF1 was immunoprecipitated from 0.2 mg SK-MEL-28 cell lysate with HA723973 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723973 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: SK-MEL-28 cell lysate (input)
Lane 2: HA723973 IP in SK-MEL-28 cell lysate
Lane 3: Rabbit IgG instead of HA723973 in SK-MEL-28 cell lysate

Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 10 seconds; ECL: K1801
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.