ABCA7 Recombinant Rabbit Monoclonal Antibody [PSH18-11]
cat.: HA723974
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH18-11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 234 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse ABCA7 aa 60-432. |
| Positive control: | Neuro-2a cell lysate, Mouse brain tissue lysate, Mouse cerebellum (P7) tissue lysate, mouse thymus tissue, rat brain tissue. |
| Subcellular location: | Cell membrane, Golgi apparatus membrane, Early endosome membrane, Cytoplasm, Cell projection, ruffle membrane, phagocytic cup. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:200 |
| Uniprot #: | SwissProt: Q91V24 Mouse | Q7TNJ2 Rat |
| Alternative names: | ABC transporter ABCA7 ABC transporter member 7 ABCA SSN ABCA-SSN ABCA7 ABCX ATP binding cassette sub family A (ABC1) member 7 ATP binding cassette sub family A member 7 ATP binding Cassette Transporter A7 ATP-binding cassette sub-family A member 7 autoantigen SS N Autoantigen SS-N FLJ40025 Macrophage ABC transporter |
Images
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Fig1:
Western blot analysis of ABCA7 on different lysates with Rabbit anti-ABCA7 antibody (HA723974) at 1/5,000 dilution. Lane 1: Neuro-2a cell lysate (no heat) (20 µg/Lane) Lane 2: Mouse brain tissue lysate (no heat) (40 µg/Lane) Lane 3: Mouse cerebellum (P7) tissue lysate (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 234 kDa Observed band size: 250 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723974) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-ABCA7 antibody (HA723974) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723974) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ABCA7 antibody (HA723974) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723974) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.