heavy chain Myosin / MYH3 Recombinant Rabbit Monoclonal Antibody [PSH18-12]
cat.: HA723975
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH18-12 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 224 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse MYH3 aa 1,811-1,860. |
| Positive control: | RD cell lysate, Mouse embryo tissue lysate, Rat embryo tissue lysate, human fetal skeletal muscle tissue, mouse E14.5 embryo tissue, rat E14.5 embryo tissue. |
| Subcellular location: | Cytoplasm, myofibril. |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:5,000-1:30,000 1-2μg/sample |
| Uniprot #: | SwissProt: P11055 Human | P13541 Mouse | P12847 Rat |
| Alternative names: | embryonic fast skeletal muscle HEMHC Muscle embryonic myosin heavy chain 3 Muscle embryonic myosin heavy chain MYH 3 Myh3 MYH3_HUMAN MYHC EMB MYHSE 1 MYHSE1 Myosin heavy chain 3 Myosin heavy chain 3 skeletal muscle embryonic Myosin heavy chain Myosin heavy chain fast skeletal muscle embryonic Myosin Heavy Polypeptide 3 Myosin heavy polypeptide 3 skeletal muscle embryonic Myosin skeletal heavy chain embryonic 1 Myosin-3 SMHCE |
Images
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Fig1:
Western blot analysis of heavy chain Myosin / MYH3 on different lysates with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution. Lane 1: RD cell lysate (20 µg/Lane) Lane 2: Mouse embryo tissue lysate (20 µg/Lane) Lane 3: Mouse testis tissue lysate (negative) (20 µg/Lane) Lane 4: Rat embryo tissue lysate (20 µg/Lane) Lane 5: Rat testis tissue lysate (negative) (20 µg/Lane) Predicted band size: 224 kDa Observed band size: 250 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723975) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse adult skeletal muscle tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat E14.5 embryo tissue with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat adult skeletal muscle tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded rat testis tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9:
heavy chain Myosin / MYH3 was immunoprecipitated from 0.2 mg RD cell lysate with HA723975 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723975 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RD cell lysate (input) Lane 2: HA723975 IP in RD cell lysate Lane 3: Rabbit IgG instead of HA723975 in RD cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 20 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.