Complement C3 Recombinant Rabbit Monoclonal Antibody [PSH18-31]
cat.: HA723984
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH18-31 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 186 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Complement C3 aa 1-1,663. |
| Positive control: | Mouse liver tissue lysate, Mouse spleen tissue lysate, Rat liver tissue lysate, Rat spleen tissue lysate, mouse liver tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-Fr IHC-P |
1:2,000 1:500 1:1,000 |
| Uniprot #: | SwissProt: P01027 Mouse | P01026 Rat |
| Alternative names: | Acylation stimulating protein cleavage product AHUS5 ARMD9 ASP C3 and PZP like alpha 2 macroglobulin domain containing protein 1 C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 C3 CO3_HUMAN Complement C3 Complement C3c alpha' chain fragment 2 Complement component 3 Complement factor 3 CPAMD1 HEL S 62p |
Images
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Fig1:
Western blot analysis of Complement C3 on different lysates with Rabbit anti-Complement C3 antibody (HA723984) at 1/2,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Rat liver tissue lysate Lane 4: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 186 kDa Observed band size: 43-186 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723984) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Application: IHC-Fr Species: Mouse Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig3:
Application: IHC-Fr Species: Rat Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Complement C3 antibody (HA723984) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723984) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.