Mouse/Rat HGF Recombinant Rabbit Monoclonal Antibody [PSH18-32]
cat.: HA723985
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH18-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse HGF aa 33-728 (HA211169). |
| Positive control: | Recombinant Mouse HGF protein (HA211169). |
| Subcellular location: | Extracellular region. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-33] to Mouse/Rat HGF antibody (Detector) (HA723986) and Recombinant Mouse/Rat HGF protein (HA211169) as the standard. The reference range value is 156-20,000 pg/mL. |
| Uniprot #: | SwissProt: Q08048 Mouse | P17945 Rat |
| Alternative names: | DFNB39 F TCF Fibroblast derived tumor cytotoxic factor Hepatocyte growth factor (hepapoietin A; scatter factor) Hepatocyte growth factor Hepatocyte growth factor beta chain Hepatocyte growth factor precursor Hepatopoietin A Hepatopoietin-A Hgf HGFB HPTA Lung fibroblast derived mitogen OTTHUMP00000161349 OTTHUMP00000206710 OTTHUMP00000206711 OTTHUMP00000206712 OTTHUMP00000206713 OTTHUMP00000206730 Scatter factor SF |
Images
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Fig1:
Sandwich ELISA analysis of Mouse/Rat HGF matched pair antibodies Capture: HA723985, Mouse/Rat HGF Rabbit mAb [PSH18-32] Detector: HA723986, Mouse/Rat HGF Rabbit mAb [PSH18-33] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723985) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse HGF protein (HA211169) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723986, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native HGF in mouse serum. Capture: HA723985, Mouse/Rat HGF Rabbit mAb [PSH18-32] Detector: HA723986, Mouse/Rat HGF Rabbit mAb [PSH18-33] Interpolated concentration of native HGF was measured in duplicate at different sample concentrations and interpolated from the HGF standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 5,959 pg/ml in mouse serum. |
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Fig3:
Interpolated concentrations of native HGF in Rat serum. Capture: HA723985, Mouse/Rat HGF Rabbit mAb [PSH18-32] Detector: HA723986, Mouse/Rat HGF Rabbit mAb [PSH18-33] Interpolated concentration of native HGF was measured in duplicate at different sample concentrations and interpolated from the HGF standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 66,277 pg/ml in Rat serum. |
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Fig4:
Interpolated concentrations of native HGF in NIH/3T3 Cell extract samples based on a 1000 µg/ml extract load. Capture: HA723985, Mouse/Rat HGF Rabbit mAb [PSH18-32] Detector: HA723986, Mouse/Rat HGF Rabbit mAb [PSH18-33] Interpolated concentration of native HGF was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 12,687 pg/ml in NIH/3T3 Cell extract. |
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Fig5:
Interpolated concentrations of spiked HGF in cell culture media samples. Capture: HA723985, Mouse/Rat HGF Rabbit mAb [PSH18-32] Detector: HA723986, Mouse/Rat HGF Rabbit mAb [PSH18-33] The concentrations of HGF were measured in duplicates, interpolated from the HGF standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.