Human/Mouse/Rat FGF1 Recombinant Rabbit Monoclonal Antibody [PSH18-34]
cat.: HA723987
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH18-34 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FGF1 aa 16-155 (HA211079). |
| Positive control: | Recombinant Human FGF1 protein (HA211079). |
| Subcellular location: | Secreted, Cytoplasm, cell cortex, cytosol, Nucleus. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-35] to Human/Mouse/Rat FGF1 antibody (Detector) (HA723988) and Recombinant Human FGF1 protein (HA211079) as the standard. The reference range value is 7.8-2,000 pg/mL. |
| Uniprot #: | SwissProt: P05230 Human | P61148 Mouse | P61149 Rat |
| Alternative names: | Acidic fibroblast growth factor aFGF Beta endothelial cell growth factor Beta-endothelial cell growth factor ECGF ECGF beta ECGF-beta ECGFA ECGFB Endothelial Cell Growth Factor alpha Endothelial Cell Growth Factor beta FGF 1 FGF alpha Fgf1 FGF1_HUMAN FGFA Fibroblast Growth Factor 1 Acidic Fibroblast growth factor 1 GLIO703 HBGF 1 HBGF-1 HBGF1 Heparin binding growth factor 1 Heparin binding growth factor 1 precursor Heparin-binding growth factor 1 |
Images
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Fig1:
Sandwich ELISA analysis of Human FGF1 matched pair antibodies Capture: HA723987, Human/Mouse/Rat FGF1 Rabbit mAb [PSH18-34] Detector: HA723988, Human/Mouse/Rat FGF1 Rabbit mAb [PSH18-35] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723987) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human FGF1 protein (HA211079) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723988, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native FGF1 in U-87 MG cell extract samples based on a 1,000 µg/ml extract load. Capture: HA723987, Human/Mouse/Rat FGF1 Rabbit mAb [PSH18-34] Detector: HA723988, Human/Mouse/Rat FGF1 Rabbit mAb [PSH18-35] The concentrations of FGF1 were measured in duplicates, interpolated from the FGF1 standard curve and corrected for sample dilution. Undiluted samples are U-87 MG cell extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF1 concentration was determined to be 337 pg/ml in U-87 MG cell extract. |
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Fig3:
Interpolated concentrations of native FGF1 in mouse brain tissue extract and rat heart tissue extract samples based on a 1,000 µg/ml extract load. Capture: HA723987, Human/Mouse/Rat FGF1 Rabbit mAb [PSH18-34] Detector: HA723988, Human/Mouse/Rat FGF1 Rabbit mAb [PSH18-35] The concentrations of FGF1 were measured in duplicates, interpolated from the FGF1 standard curve and corrected for sample dilution. Undiluted samples are mouse brain tissue extract 5% and rat heart tissue extract 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF1 concentration was determined to be 3,420 pg/ml in mouse brain tissue extract and 7,048 pg/ml in rat heart tissue extract. |
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Fig4:
Interpolated concentrations of spiked FGF1 in human cell culture media samples. Capture: HA723987, Human/Mouse/Rat FGF1 Rabbit mAb [PSH18-34] Detector: HA723988, Human/Mouse/Rat FGF1 Rabbit mAb [PSH18-35] The concentrations of FGF1 were measured in duplicates, interpolated from the FGF1 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.