Rat IL-1 alpha Recombinant Rabbit Monoclonal Antibody [PSH18-36]
cat.: HA723989
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH18-36 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Rat IL-1 alpha aa 115-270 (HA211058). |
| Positive control: | Recombinant Rat IL-1 alpha protein (HA211058). |
| Subcellular location: | Nucleus, Cytoplasm, Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-37] to Rat IL-1 alpha antibody (Detector) (HA723990) and Recombinant Rat IL-1 alpha protein (HA211058) as the standard. The reference range value is 3.9-1,000 pg/mL. |
| Uniprot #: | SwissProt: P16598 Rat |
| Alternative names: | BAF FAF Hematopoietin 1 Hematopoietin-1 IL 1 alpha IL 1A IL-1 alpha Il-1a IL1 ALPHA IL1 IL1A IL1F1 Interleukin 1 alpha Interleukin-1 alpha Interleukin1 alpha LAF LEM Preinterleukin 1 alpha Pro interleukin 1 alpha |
Images
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Fig1:
Sandwich ELISA analysis of rat IL-1α matched pair antibodies Capture: HA723989, Rat IL-1 alpha Rabbit mAb [PSH18-36] Detector: HA723990, Rat IL-1 alpha Rabbit mAb [PSH18-37] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723989) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat IL-1 alpha protein (HA211058) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723990, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Rat spleen cells were cultured unstimulated or stimulated with 100 ng/mL LPS. Capture: HA723989, Rat IL-1 alpha Rabbit mAb [PSH18-36] Detector: HA723990, Rat IL-1 alpha Rabbit mAb [PSH18-37] Conditioned media was harvested after 72 hours. IL-1α was measured in 100% unstimulated and LPS stimulated rat spleen cell supernatant. The concentrations of IL-1α were interpolated from the IL-1α standard curves. The mean IL-1α concentration was determined to be 26 pg/mL in LPS stimulated rat spleen cell supernatant. There was no detectable signal in unstimulated supernatant. |
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Fig3:
Interpolated concentrations of spiked IL-1α in cell culture media samples. Capture: HA723989, Rat IL-1 alpha Rabbit mAb [PSH18-36] Detector: HA723990, Rat IL-1 alpha Rabbit mAb [PSH18-37] The concentrations of IL-1α were measured in duplicates, interpolated from the IL-1α standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.