Human/Mouse/Rat Synapsin I Recombinant Rabbit Monoclonal Antibody [PSH18-40]
cat.: HA723993
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH18-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Synapsin I aa 1-705 (HA211359). |
| Positive control: | Recombinant Human Synapsin I protein (HA211359). |
| Subcellular location: | Synapse, Golgi apparatus, Presynapse, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-41] to Human/Mouse/Rat Synapsin I antibody (Detector) (HA723994) and Recombinant Human Synapsin I protein (HA211359) as the standard. The reference range value is 187-48,000 pg/mL. |
| Uniprot #: | SwissProt: P17600 Human | O88935 Mouse | P09951 Rat |
| Alternative names: | Brain protein 4.1 SYN 1 SYN 1a SYN 1b SYN I SYN1 SYN1_HUMAN SYN1a SYN1b Synapsin 1 Synapsin I Synapsin-1 Synapsin1 SynapsinI SYNI Phospho-Synapsin Ⅰ (S9) Phospho Synapsin Ⅰ (S9) Phospho-Synapsin Ⅰ (S9) |
Images
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Fig1:
Sandwich ELISA analysis of human Synapsin 1 matched pair antibodies Capture: HA723993, Human/Mouse/Rat Synapsin I Rabbit mAb [PSH18-40] Detector: HA723994, Human/Mouse/Rat Synapsin I Rabbit mAb [PSH18-41] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723993) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Synapsin I protein (HA211359) starting from 48,000 pg/ml to 0 pg/ml and detect antibody (HA723994, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Synapsin-1 in SH-SY5Y cell extract, mouse brain and mouse lung tissue extract samples based on a 1000 µg/ml extract load. Capture: HA723993, Human/Mouse/Rat Synapsin I Rabbit mAb [PSH18-40] Detector: HA723994, Human/Mouse/Rat Synapsin I Rabbit mAb [PSH18-41] Interpolated concentration of native Synapsin-1 was measured in duplicate at different sample concentrations and interpolated from the Synapsin-1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Synapsin-1 concentration was determined to be 7.428 ug/mL in mouse brain tissue extract and 110 ng/mL in SH-SY5Y cell extract. There was no detectable signal in mouse lung tissue extract. |
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Fig3:
Interpolated concentrations of native Synapsin-1 in rat brain tissue extract samples based on a 1000 µg/ml extract load. Capture: HA723993, Human/Mouse/Rat Synapsin I Rabbit mAb [PSH18-40] Detector: HA723994, Human/Mouse/Rat Synapsin I Rabbit mAb [PSH18-41] Interpolated concentration of native Synapsin-1 was measured in duplicate at different sample concentrations and interpolated from the Synapsin-1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Synapsin-1 concentration was determined to be 6.329 ug/mL in rat brain tissue extract. |
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Fig4:
Interpolated concentrations of spiked Synapsin-1 in cell culture media samples. Capture: HA723993, Human/Mouse/Rat Synapsin I Rabbit mAb [PSH18-40] Detector: HA723994, Human/Mouse/Rat Synapsin I Rabbit mAb [PSH18-41] The concentrations of Synapsin-1 were measured in duplicates, interpolated from the Synapsin-1 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
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Fig5:
Sandwich ELISA analysis of human Synapsin 1 matched pair antibodies Capture: HA723993, Human/Mouse/Rat Synapsin I Rabbit mAb [PSH18-40] Detector: HA723994, Human/Mouse/Rat Synapsin I Rabbit mAb [PSH18-41] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723993) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Synapsin I (HA211359) or Human Synapsin 2 protein starting from 48,000 pg/ml to 0 pg/ml and detect antibody (HA723994, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.