Human AHNAK Recombinant Rabbit Monoclonal Antibody [PSH18-43]
cat.: HA723996
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH18-43 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human AHNAK aa 483-881 (HA210552). |
| Positive control: | Recombinant Human AHNAK protein (HA210552). |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-42] to Human AHNAK antibody (Capture) (HA723995) and Recombinant Human AHNAK protein (HA210552) as the standard. The reference range value is 187-48,000 pg/mL. |
| Uniprot #: | SwissProt: Q09666 Human |
| Alternative names: | AHNAK AHNAK nucleoprotein (desmoyokin) AHNAK nucleoprotein AHNAKRS AHNK_HUMAN Desmoyokin Fragments MGC5395 Neuroblast differentiation associated protein AHNAK Neuroblast differentiation-associated protein AHNAK PM227 |
Images
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Fig1:
Sandwich ELISA analysis of human AHNAK matched pair antibodies Capture: HA723995, Human AHNAK Rabbit mAb [PSH18-42] Detector: HA723996, Human AHNAK Rabbit mAb [PSH18-43] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723995) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human AHNAK protein (HA210552) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723996, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native AHNAK in Hela, A431 and Hep G2 extract samples based on a 1000 µg/ml extract load. Capture: HA723995, Human AHNAK Rabbit mAb [PSH18-42] Detector: HA723996, Human AHNAK Rabbit mAb [PSH18-43] Interpolated concentration of native AHNAK was measured in duplicate at different sample concentrations and interpolated from the AHNAK standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean AHNAK concentration was determined to be 52,903 pg/mL in Hela, 40,872 pg/mL in A431 and 3.387 pg/mL in Hep G2 cell extract. |
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Fig3:
Interpolated concentrations of spiked AHNAK in cell culture media samples. Capture: HA723995, Human AHNAK Rabbit mAb [PSH18-42] Detector: HA723996, Human AHNAK Rabbit mAb [PSH18-43] The concentrations of AHNAK were measured in duplicates, interpolated from the AHNAK standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.