ADAMTS1 Recombinant Rabbit Monoclonal Antibody [PSH18-51]
cat.: HA724004
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH18-51 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 105 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ADAMTS1 aa 701-967. |
| Positive control: | HeLa cell lysate, HeLa treated with 10μM BFA for 24 hours cell lysate, Rat kidney tissue lysate, Mouse kidney tissue lysate, Mouse brain tissue lysate, 4T1 cell lysate, SK-OV-3 cell lysate, NCI-H226 cell lysate. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q9UHI8 Human |
| Alternative names: | A disintegrin and metalloproteinase with thrombospondin motifs 1 ADAM-TS 1 ADAM-TS1 ADAMTS 1 ADAMTS ADAMTS-1 Adamts1 ATS1_HUMAN C3 C5 KIAA1346 METH 1 METH-1 |
Images
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Fig1:
Western blot analysis of ADAMTS1 on different lysates with Rabbit anti-ADAMTS1 antibody (HA724004) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10μM BFA for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724004) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ADAMTS1 on different lysates with Rabbit anti-ADAMTS1 antibody (HA724004) at 1/5,000 dilution. Lane 1: Rat kidney tissue lysate (40 µg/Lane) Lane 2: Mouse kidney tissue lysate (40 µg/Lane) Lane 3: Mouse brain tissue lysate (low expression) (40 µg/Lane) Lane 4: 4T1 cell lysate (20 µg/Lane) Lane 5: SK-OV-3 cell lysate (20 µg/Lane) Lane 6: NCI-H226 cell lysate (20 µg/Lane) Lane 7: HeLa cell lysate (20 µg/Lane) Predicted band size: 105 kDa Observed band size: 105/90 kDa Exposure time: 2 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724004) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.