BACH1 Recombinant Rabbit Monoclonal Antibody [PSH18-52]
cat.: HA724005
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC, IP, ChIP |
| Clonality: | Monoclonal |
| Clone number: | PSH18-52 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 82 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human BACH1 aa 100-580. |
| Positive control: | Jurkat cell lysate, MCF7 cell lysate, PANC-1 cell lysate, TF-1 cell lysate, BxPC-3 cell lysate, HEK-293. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC IP ChIP |
1:5,000 1:1,000 1-2μg/sample Use 5 μg for 25 μg of chromatin. |
| Uniprot #: | SwissProt: O14867 Human |
| Alternative names: | BACH 1 BACH 1.3 Bach1 BACH1_HUMAN Basic leucine zipper transcription factor 1 basic region leucine zipper transcriptional regulator BACH1 BTB and CNC homolog 1 BTB and CNC homology 1 BTB and CNC homology 1 basic leucine zipper transcription factor 1 HA2303 OTTHUMP00000096563 OTTHUMP00000096564 Transcription regulator protein BACH1 |
Images
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Fig1:
Western blot analysis of BACH1 on different lysates with Rabbit anti-BACH1 antibody (HA724005) at 1/5,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: PANC-1 cell lysate (20 µg/Lane) Lane 4: TF-1 cell lysate (20 µg/Lane) Lane 5: BxPC-3 cell lysate (20 µg/Lane) Predicted band size: 82 kDa Observed band size: 90 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724005) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Flow cytometric analysis of HEK-293 cells labeling BACH1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA724005, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig3:
BACH1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA724005 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724005 at 1/5,000 dilution. Anti-rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA724005 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA724005 in Jurkat cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 10 seconds; ECL: K1801 |
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Fig4: Chromatin immunoprecipitations were performed with cross-linked chromatin from Jurkat cells with BACH1 (HA724005) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.