TREM2 Recombinant Rabbit Monoclonal Antibody [PSH18-55]
cat.: HA724008
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH18-55 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse TREM2 aa 111-160. |
| Positive control: | J774A.1 cell lysate, RAW264.7 cell lysate. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
WB IP |
1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q99NH8 Mouse |
| Alternative names: | TREM 2 TREM-2 TREM2 TREM2a TREM2b TREM2c Trggering receptor expressed on myeloid cells 2 Trggering receptor expressed on myeloid cells 2a Triggering receptor expressed on monocytes 2 Triggering receptor expressed on myeloid cells 2 |
Images
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Fig1:
Western blot analysis of TREM2 on different lysates with Rabbit anti-TREM2 antibody (HA724008) at 1/5,000 dilution. Lane 1: J774A.1 cell lysate Lane 2: Neuro-2a cell lysate (negative) Lane 3: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 30 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724008) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
TREM2 was immunoprecipitated from 0.2 mg J774A.1 cell lysate with HA724008 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724008 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: J774A.1 cell lysate (input) Lane 2: HA724008 IP in J774A.1 cell lysate Lane 3: Rabbit IgG instead of HA724008 in J774A.1 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 2 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.