Cystatin C Recombinant Rabbit Monoclonal Antibody [PSH18-60]
cat.: HA724013
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH18-60 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 16 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Cystatin C aa 1-146. |
| Positive control: | MDA-MB-231 cell lysate, Huh7 cell lysate, TT cell lysate, human kidney tissue, human ovary tissue, mouse kidney tissue, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Secreted |
| Recommended Dilutions:
WB IHC-P |
1:20,000 1:2,000-1:5,000 |
| Uniprot #: | SwissProt: P01034 Human | P21460 Mouse | P14841 Rat |
| Alternative names: | Cystatin-C Cystatin-3 Gamma-trace Neuroendocrine basic polypeptide Post-gamma-globulin CST3 |
Images
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Fig1:
Western blot analysis of Cystatin C on different lysates with Rabbit anti-Cystatin C antibody (HA724013) at 1/20,000 dilution. Lane 1: MDA-MB-231 cell lysate (20 µg/Lane) Lane 2: MOLT-4 cell lysate (negative) (20 µg/Lane) Lane 3: Huh7 cell lysate (20 µg/Lane) Lane 4: TT cell lysate (20 µg/Lane) Predicted band size: 16 kDa Observed band size: 14 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724013) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cystatin C antibody (HA724013) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724013) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-Cystatin C antibody (HA724013) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724013) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Cystatin C antibody (HA724013) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724013) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Western blot analysis of Cystatin C on different lysates with Rabbit anti-Cystatin C antibody (HA724013) at 1/20,000 dilution. Lane 1: Mouse brain tissue lysate (10 µg/Lane) Lane 2: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 16 kDa Observed band size: 16/18 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724013) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.