Human Fibromodulin Recombinant Rabbit Monoclonal Antibody [PSH18-72]
cat.: HA724015
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH18-72 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Fibromodulin aa 19-376 (HA211358). |
| Positive control: | Recombinant Human Fibromodulin protein (HA211358). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-73] to Human Fibromodulin antibody (Detector) (HA724016) and Recombinant Human Fibromodulin protein (HA211358) as the standard. The reference range value is 19.5-5,000 pg/mL. |
| Uniprot #: | SwissProt: Q06828 Human |
| Alternative names: | Fibromodulin FM Collagen-binding 59 kDa protein Keratan sulfate proteoglycan fibromodulin KSPG fibromodulin FMOD SLRR2E |
Images
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Fig1:
Sandwich ELISA analysis of Human Fibromodulin matched pair antibodies Capture: HA724015, Human Fibromodulin Rabbit mAb [PSH18-72] Detector: HA724016, Human Fibromodulin Rabbit mAb [PSH18-73] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724015) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Fibromodulin protein (HA211358) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA724016, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Fibromodulin in human serum samples. Capture: HA724015, Human Fibromodulin Rabbit mAb [PSH18-72] Detector: HA724016, Human Fibromodulin Rabbit mAb [PSH18-73] The concentrations of Fibromodulin were measured in triplicates, interpolated from the Fibromodulin standard curve and corrected for sample dilution. Undiluted samples are human serum 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean Fibromodulin concentration was determined to be 2,988 pg/ml in human serum. |
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Fig3:
Interpolated concentrations of native Fibromodulin in Caco-2 and K-562 cell culture supernatant. Capture: HA724015, Human Fibromodulin Rabbit mAb [PSH18-72] Detector: HA724016, Human Fibromodulin Rabbit mAb [PSH18-73] The concentrations of Fibromodulin were measured in duplicates, interpolated from the Fibromodulin standard curve and corrected for sample dilution. Undiluted samples are Caco-2 cell culture supernatant 50% and K-562 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibromodulin concentration was determined to be 1,510 pg/ml in Caco-2 cell culture supernatant and undetectable in K-562 cell culture supernatant. |
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Fig4:
Interpolated concentrations of spiked Fibromodulin in human cell culture media samples. Capture: HA724015, Human Fibromodulin Rabbit mAb [PSH18-72] Detector: HA724016, Human Fibromodulin Rabbit mAb [PSH18-73] The concentrations of Fibromodulin were measured in duplicates, interpolated from the Fibromodulin standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.