Human Apolipoprotein A1 Recombinant Rabbit Monoclonal Antibody [PSH18-89]
cat.: HA724026
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH18-89 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Apolipoprotein A1 aa 25-267 (HA211090). |
| Positive control: | Recombinant Human Apolipoprotein A1 protein (HA211090). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-90] to Human Apolipoprotein A1 antibody (Detector) (HA724027) and Recombinant Human Apolipoprotein A1 protein (HA211090) as the standard. The reference range value is 234-15,000 pg/mL. |
| Uniprot #: | SwissProt: P02647 Human |
| Alternative names: | Apo-AI ApoA I ApoA-I APOA1 APOA1_HUMAN Apolipoprotein A-I(1-242) Apolipoprotein A1 Apolipoprotein AI Brp14 Ltw1 Lvtw1 Sep1 Sep2 |
Images
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Fig1:
Sandwich ELISA analysis of Human Apolipoprotein A1 matched pair antibodies Capture: HA724026, Human Apolipoprotein A1 Rabbit mAb [PSH18-89] Detector: HA724027, Human Apolipoprotein A1 Rabbit mAb [PSH18-90] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724026) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Apolipoprotein A1 protein (HA211090) starting from 15000 pg/ml to 0 pg/ml and detect antibody (HA724027, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native ApoA1 in human samples. Capture: HA724026, Human Apolipoprotein A1 Rabbit mAb [PSH18-89] Detector: HA724027, Human Apolipoprotein A1 Rabbit mAb [PSH18-90] Interpolated concentration of native ApoA1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ApoA1 concentration was determined to be 1.26 mg/mL in human serum and 526 ng/ml in HepG2 cell culture supernatant. There was no detectable signal in HEK-293 cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked ApoA1 in cell culture media samples. Capture: HA724026, Human Apolipoprotein A1 Rabbit mAb [PSH18-89] Detector: HA724027, Human Apolipoprotein A1 Rabbit mAb [PSH18-90] The concentrations of ApoA1 were measured in duplicates, interpolated from the ApoA1 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.