Human PIGR Recombinant Rabbit Monoclonal Antibody [PSH18-94]
cat.: HA724031
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH18-94 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PIGR aa 19-638 (HA211360). |
| Positive control: | Recombinant Human PIGR protein (HA211360). |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-92] to Human PIGR antibody (Capture) (HA724029) and Recombinant Human PIGR protein (HA211360) as the standard. The reference range value is 78.1-20,000 pg/mL. |
| Uniprot #: | SwissProt: P01833 Human |
| Alternative names: | Hepatocellular carcinoma associated protein TB6 Hepatocellular carcinoma-associated protein TB6 MGC125361 MGC125362 Phosphatidylinositol glycan, class R PIGR PIGR_HUMAN Poly Ig receptor Poly-Ig receptor Polymeric immunoglobulin receptor Secretory component Transmembrane secretory component |
Images
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Fig1:
Sandwich ELISA analysis of Human PIGR matched pair antibodies Capture: HA724029, Human PIGR Rabbit mAb [PSH18-92] Detector: HA724031, Human PIGR Rabbit mAb [PSH18-94] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724029) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PIGR protein (HA211360) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA724031, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native PIGR in Human serum. Capture: HA724029, Human PIGR Rabbit mAb [PSH18-92] Detector: HA724031, Human PIGR Rabbit mAb [PSH18-94] Interpolated concentration of native PIGR was measured in duplicate at different sample concentrations and interpolated from the PIGR standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean PIGR concentration was determined to be 487,829 pg/ml in Human serum. |
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Fig3:
Interpolated concentrations of native PIGR in A431 cell culture supernatant. Capture: HA724029, Human PIGR Rabbit mAb [PSH18-92] Detector: HA724031, Human PIGR Rabbit mAb [PSH18-94] Interpolated concentration of native PIGR was measured in duplicate at different sample concentrations and interpolated from the PIGR standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean PIGR concentration was determined to be 10,525 pg/ml in A431 cell culture supernatant and undetectable in Daudi cell culture supernatant. |
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Fig4:
Interpolated concentrations of spiked PIGR in cell culture media samples. Capture: HA724029, Human PIGR Rabbit mAb [PSH18-92] Detector: HA724031, Human PIGR Rabbit mAb [PSH18-94] The concentrations of PIGR were measured in duplicates, interpolated from the PIGR standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.