FOXL2 Recombinant Rabbit Monoclonal Antibody [PSH19-02]
cat.: HA724038
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: PSH19-02
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 39 kDa
Isotype: IgG
Immunogen: Synthetic peptide within mouse FOXL2 aa 320-375.
Positive control: SiHa cell lysate, THP-1 cell lysate, K-562 cell lysate, Mouse ovary tissue lysate, Rat ovary tissue lysate, human sex cord-stromal tumor tissue, human ovarian granulosa cell tumor tissue, human ovary tissue, mouse endometrium tissue, mouse ovary tissue, mouse oviduct tissue, rat ovary tissue, rat oviduct tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IP
1:5,000
1:200-1:1,000
1-2μg/sample
Uniprot #: SwissProt: P58012 Human | O88470 Mouse
Alternative names: Blepharophimosis Blepharophimosis epicanthus inversus and ptosis 1 Blepharophimosis epicanthus inversus and ptosis BPES 1 BPES BPES1 Epicanthus inversus and ptosis 1 Forkhead box L2 Forkhead box protein L2 Forkhead transcription factor FOXL2 FOX L2 FOXL 2 FOXL2 FOXL2_HUMAN PFRK PINTO PITUITARY FORKHEAD FACTOR POF 3 POF3
Images
HA724038_1.jpg Fig1: Western blot analysis of FOXL2 on different lysates with Rabbit anti-FOXL2 antibody (HA724038) at 1/5,000 dilution.

Lane 1: SiHa cell lysate (20 µg/Lane)
Lane 2: THP-1 cell lysate (20 µg/Lane)
Lane 3: K-562 cell lysate (20 µg/Lane)
Lane 4: Mouse ovary tissue lysate (30 µg/Lane)
Lane 5: Mouse testis tissue lysate (negative) (30 µg/Lane)
Lane 6: Rat ovary tissue lysate (30 µg/Lane)
Lane 7: Rat testis tissue lysate (negative) (30 µg/Lane)

Predicted band size: 39 kDa
Observed band size: 50 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724038) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA724038_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human sex cord-stromal tumor tissue with Rabbit anti-FOXL2 antibody (HA724038) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724038) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA724038_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human ovarian granulosa cell tumor tissue with Rabbit anti-FOXL2 antibody (HA724038) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724038) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA724038_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-FOXL2 antibody (HA724038) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724038) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA724038_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue (negative) with Rabbit anti-FOXL2 antibody (HA724038) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724038) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA724038_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human testis tissue (negative) with Rabbit anti-FOXL2 antibody (HA724038) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724038) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA724038_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse endometrium tissue with Rabbit anti-FOXL2 antibody (HA724038) at 1/5000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724038) at 1/5000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA724038_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-FOXL2 antibody (HA724038) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724038) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA724038_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse oviduct tissue with Rabbit anti-FOXL2 antibody (HA724038) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724038) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA724038_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded mouse testis tissue (negative) with Rabbit anti-FOXL2 antibody (HA724038) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724038) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA724038_11.jpg Fig11: Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-FOXL2 antibody (HA724038) at 1/1000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724038) at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA724038_12.jpg Fig12: Immunohistochemical analysis of paraffin-embedded rat oviduct tissue with Rabbit anti-FOXL2 antibody (HA724038) at 1/1000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724038) at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA724038_13.jpg Fig13: Immunohistochemical analysis of paraffin-embedded rat testis tissue (negative) with Rabbit anti-FOXL2 antibody (HA724038) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724038) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA724038_14.jpg Fig14: FOXL2 was immunoprecipitated from 0.2 mg K-562 cell lysate with HA724038 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724038 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: K-562 cell lysate (input)
Lane 2: HA724038 IP in K-562 cell lysate
Lane 3: Rabbit IgG instead of HA724038 in K-562 cell lysate

Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 46 seconds; ECL: K1801
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.