Mouse Complement Factor D Recombinant Rabbit Monoclonal Antibody [PSH19-06]
cat.: HA724040
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH19-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Complement Factor D aa 26-259 (HA211116). |
| Positive control: | Recombinant Mouse Complement Factor D protein (HA211116). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-07] to Mouse Complement Factor D antibody (Detector) (HA724041) or Rabbit monoclonal [PSH19-08] to Mouse Complement Factor D antibody (Detector) (HA724042) and Recombinant Mouse Complement Factor D protein (HA211116) as the standard. The reference range value is 23.4-6,000 pg/mL. |
| Uniprot #: | SwissProt: P03953 Mouse |
| Alternative names: | Adipsin Adipsin/complement factor D Adn C3 convertase activator CFAD_HUMAN CFD Complement factor D complement factor D preproprotein D component of complement DF FactorD PFD Properdin factor D |
Images
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Fig1:
Sandwich ELISA analysis of Mouse Complement Factor D matched pair antibodies Capture: HA724040, Mouse Complement Factor D Rabbit mAb [PSH19-06] Detector: HA724041, Mouse Complement Factor D Rabbit mAb [PSH19-07] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724040) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Complement Factor D protein (HA211116) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA724041, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Sandwich ELISA analysis of Mouse Complement Factor D matched pair antibodies Capture: HA724040, Mouse Complement Factor D Rabbit mAb [PSH19-06] Detector: HA724042, Mouse Complement Factor D Rabbit mAb [PSH19-08] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724040) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Complement Factor D protein (HA211116) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA724042, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig3:
Interpolated concentrations of native Adipsin in mouse serum samples. Capture: HA724040, Mouse Complement Factor D Rabbit mAb [PSH19-06] Detector: HA724041, Mouse Complement Factor D Rabbit mAb [PSH19-07] The concentrations of Adipsin were measured in triplicates, interpolated from the Adipsin standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.005%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean Adipsin concentration was determined to be 42.3 µg/ml in mouse serum. |
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Fig4:
Interpolated concentrations of native Adipsin in mouse serum samples. Capture: HA724040, Mouse Complement Factor D Rabbit mAb [PSH19-06] Detector: HA724042, Mouse Complement Factor D Rabbit mAb [PSH19-08] The concentrations of Adipsin were measured in duplicates, interpolated from the Adipsin standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.005%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Adipsin concentration was determined to be 12.4 µg/ml in mouse serum. |
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Fig5:
Interpolated concentrations of spiked Adipsin in mouse cell culture media samples. Capture: HA724040, Mouse Complement Factor D Rabbit mAb [PSH19-06] Detector: HA724041, Mouse Complement Factor D Rabbit mAb [PSH19-07] The concentrations of Adipsinwere measured in duplicates, interpolated from the Adipsin standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
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Fig6:
Interpolated concentrations of spiked Adipsin in mouse cell culture media samples. Capture: HA724040, Mouse Complement Factor D Rabbit mAb [PSH19-06] Detector: HA724042, Mouse Complement Factor D Rabbit mAb [PSH19-08] The concentrations of Adipsinwere measured in duplicates, interpolated from the Adipsin standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.