HRP Conjugated Mouse Complement Factor D Recombinant Rabbit Monoclonal Antibody [PSH19-08]
cat.: HA724042H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH19-08 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Complement Factor D aa 26-259 (HA211116). |
| Positive control: | Recombinant Mouse Complement Factor D protein (HA211116). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-06] to Mouse Complement Factor D antibody (Capture) (HA724040) and Recombinant Mouse Complement Factor D protein (HA211116) as the standard. The reference range value is 46.88-6,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P03953 Mouse |
| Alternative names: | Adipsin Adipsin/complement factor D Adn C3 convertase activator CFAD_HUMAN CFD Complement factor D complement factor D preproprotein D component of complement DF FactorD PFD Properdin factor D |
Images
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Fig1:
Sandwich ELISA analysis of Mouse Complement Factor D matched pair antibodies Capture: HA724040, Mouse Complement Factor D Rabbit mAb [PSH19-06] Detector: HA724042, Mouse Complement Factor D Rabbit mAb [PSH19-08] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724040) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Complement Factor D protein (HA211116) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA724042, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.