Human EGFR Recombinant Rabbit Monoclonal Antibody [PSH19-09]
cat.: HA724043
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap), ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH19-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human EGFR aa 25-645 (HA211104). |
| Positive control: | Recombinant Human EGFR protein (HA211104). |
| Subcellular location: | Cell membrane, Endoplasmic reticulum membrane, Golgi apparatus membrane, Nucleus membrane, Endosome, Endosome membrane, Nucleus; Secreted. |
| Recommended Dilutions:
ELISA(Cap) ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-10] to Human EGFR antibody (Detector) (HA724044) and Recombinant Human EGFR protein (HA211104) as the standard. The reference range value is 78.1-20,000 pg/mL. Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-11] to Human EGFR antibody (Capture) (HA724045) and Recombinant Human EGFR protein (HA211104) as the standard. The reference range value is 78.1-20,000 pg/mL. |
| Uniprot #: | SwissProt: P00533 Human |
| Alternative names: | Avian erythroblastic leukemia viral (v erb b) oncogene homolog Cell growth inhibiting protein 40 Cell proliferation inducing protein 61 EGF R EGFR EGFR_HUMAN Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) Epidermal growth factor receptor erb-b2 receptor tyrosine kinase 1 ERBB ERBB1 Errp HER1 mENA NISBD2 Oncogen ERBB PIG61 Proto-oncogene c-ErbB-1 Receptor tyrosine protein kinase ErbB 1 Receptor tyrosine-protein kinase ErbB-1 SA7 Species antigen 7 Urogastrone v-erb-b Avian erythroblastic leukemia viral oncogen homolog wa2 Wa5 |
Images
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Fig1:
Sandwich ELISA analysis of Human EGFR matched pair antibodies Capture: HA724043, Human EGFR Rabbit mAb [PSH19-09] Detector: HA724044, Human EGFR Rabbit mAb [PSH19-10] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724043) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human EGFR protein (HA211104) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA724044, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native EGFR in A431 and Jurkat cell culture supernatant. Capture: HA724043, Human EGFR Rabbit mAb [PSH19-09] Detector: HA724044, Human EGFR Rabbit mAb [PSH19-10] Interpolated concentration of native EGFR was measured in duplicate at different sample concentrations and interpolated from the EGFR standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean EGFR concentration was determined to be 84,020 pg/ml in A431 cell culture supernatant and undetectable in Jurkat cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked EGFR in cell culture media samples. Capture: HA724043, Human EGFR Rabbit mAb [PSH19-09] Detector: HA724044, Human EGFR Rabbit mAb [PSH19-10] The concentrations of EGFR were measured in duplicates, interpolated from the EGFR standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
|
Fig4:
Sandwich ELISA analysis of Human EGFR matched pair antibodies Capture: HA724045, Human EGFR Rabbit mAb [PSH19-11] Detector: HA724043, Human EGFR Rabbit mAb [PSH19-09] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724045) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human EGFR protein (HA211104) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA724043, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig5:
Interpolated concentrations of native EGFR in A431 and Jurkat cell culture supernatant. Capture: HA724045, Human EGFR Rabbit mAb [PSH19-11] Detector: HA724043, Human EGFR Rabbit mAb [PSH19-09] Interpolated concentration of native EGFR was measured in duplicate at different sample concentrations and interpolated from the EGFR standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean EGFR concentration was determined to be 133,426 pg/ml in A431 cell culture supernatant and undetectable in Jurkat cell culture supernatant. |
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Fig6:
Interpolated concentrations of spiked EGFR in cell culture media samples. Capture: HA724045, Human EGFR Rabbit mAb [PSH19-11] Detector: HA724043, Human EGFR Rabbit mAb [PSH19-09] The concentrations of EGFR were measured in duplicates, interpolated from the EGFR standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.