Dystrophin Recombinant Rabbit Monoclonal Antibody [PSH19-15]
cat.: HA724049
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, WB |
| Clonality: | Monoclonal |
| Clone number: | PSH19-15 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 426 kDa |
| Isotype: | IgG |
| Positive control: | Mouse heart tissue, mouse colon tissue, rat heart tissue, rat colon tissue, Mouse heart tissue lysate, Mouse skeletal muscle tissue lysate, Rat heart tissue lysate, Rat skeletal muscle tissue lysate. |
| Subcellular location: | Cell membrane, sarcolemma, Cytoplasm, cytoskeleton, Postsynaptic cell membrane. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:500 1:500-1:2,000 1:10,000 |
| Uniprot #: | SwissProt: P11531 Mouse | P11530 Rat |
| Alternative names: | BMD CMD3B DMD DMD_HUMAN Duchenne muscular dystrophy protein Dystrophin Muscular dystrophy Duchenne and Becker types |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: heart Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Rat Site: heart Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Dystrophin antibody (HA724049) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724049) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Dystrophin antibody (HA724049) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724049) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-Dystrophin antibody (HA724049) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724049) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Dystrophin antibody (HA724049) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724049) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Dystrophin antibody (HA724049) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724049) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig8:
Western blot analysis of Dystrophin on different lysates with Rabbit anti-Dystrophin antibody (HA724049) at 1/10,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Mouse skeletal muscle tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lane 4: Rat heart tissue lysate Lane 5: Rat skeletal muscle tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Exposure time: 40 seconds; ECL: K1802; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724049) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.