VEGFA Recombinant Rabbit Monoclonal Antibody [PSH19-17]
cat.: HA724051
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH19-17 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Positive control: | U-87 MG cell lysate, NCI-H441 cell lysate, EA.hy926 cell lysate, SH-SY5Y cell lysate, MDA-MB-231 cell lysate, A431 cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, bEnd.3 cell lysate, RAW264.7 cell lysate, C6 cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P15692 Human | Q00731 Mouse | P16612 Rat |
| Alternative names: | VEGF VEGFA L-VEGF Vascular permeability factor VPF |
Images
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Fig1:
Western blot analysis of VEGFA on different lysates with Rabbit anti-VEGFA antibody (HA724051) at 1/5,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: NCI-H441 cell lysate Lane 3: EA.hy926 cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: MDA-MB-231 cell lysate Lane 6: A431 cell lysate Lane 7: COS-1 cell lysate Lane 8: Neuro-2a cell lysate Lane 9: bEnd.3 cell lysate Lane 10: RAW264.7 cell lysate Lane 11: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724051) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.