Serotonin transporter Recombinant Rabbit Monoclonal Antibody [PSH19-18]
cat.: HA724052
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, WB |
| Clonality: | Monoclonal |
| Clone number: | PSH19-18 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG |
| Positive control: | Mouse brain tissue, mouse spleen tissue, rat brain tissue, rat spleen tissue. |
| Subcellular location: | Cell membrane, Endomembrane system, Endosome membrane, Synapse, Cell junction, focal adhesion, Cell projection, neuron projection. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:500 1:2,000 1:2,000 |
| Uniprot #: | SwissProt: Q60857 Mouse | P31652 Rat |
| Alternative names: | 5 HTT 5 HTTLPR 5 hydroxytryptamine (serotonin) transporter 5 hydroxytryptamine transporter 5HT transporter 5HTT hSERT HTT Na+/Cl- dependent serotonin transporter OCD1 SC6A4_HUMAN Serotonin transporter 1 SERT SERT1 Slc6a4 Sodium dependent serotonin transporter Sodium-dependent serotonin transporter Solute carrier family 6 (neurotransmitter transporter) member 4 solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 Solute carrier family 6 member 4 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Mouse Site: spleen Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig3:
Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig4:
Application: IHC-Fr Species: Rat Site: spleen Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Serotonin transporter antibody (HA724052) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724052) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Serotonin transporter antibody (HA724052) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724052) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue (negative) with Rabbit anti-Serotonin transporter antibody (HA724052) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724052) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig8:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Serotonin transporter antibody (HA724052) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724052) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig9:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Serotonin transporter antibody (HA724052) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724052) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig10:
Immunohistochemical analysis of paraffin-embedded rat lung tissue (negative) with Rabbit anti-Serotonin transporter antibody (HA724052) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724052) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig11:
Western blot analysis of Serotonin transporter on different lysates with Rabbit anti-Serotonin transporter antibody (HA724052) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse lung tissue lysate (negative) Lane 3: Rat brain tissue lysate Lane 4: Rat lung tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Notice: unboiled means the lysate is not boiled. Predicted band size: 70 kDa Observed band size: ~70 kDa Exposure time: 78 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724052) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.