MMP-13 Recombinant Rabbit Monoclonal Antibody [PSH19-21]
cat.: HA724055
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH19-21 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse MMP-13 aa 1-472 / 472. |
| Positive control: | Mouse articular cartilage tissue lysate, Mouse embryo tissue lysate, mouse knee joint tissue. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:10,000 |
| Uniprot #: | SwissProt: P33435 Mouse |
| Alternative names: | CLG 3 CLG3 Collagenase 3 Collagenase3 MANDP1 Matrix metallopeptidase 13 (collagenase 3) Matrix Metalloproteinase 13 Matrix metalloproteinase-13 MMP 13 MMP-13 Mmp13 |
Images
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Fig1:
Western blot analysis of MMP-13 on different lysates with Rabbit anti-MMP-13 antibody (HA724055) at 1/5,000 dilution. Lane 1: Mouse articular cartilage tissue lysate (40 µg/Lane) Lane 2: Mouse skeletal muscle tissue lysate (negative) (40 µg/Lane) Lane 3: Mouse embryo tissue lysate (40 µg/Lane) Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: Lane 1: 30 seconds; Lane 2-3: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724055) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse knee joint tissue with Rabbit anti-MMP-13 antibody (HA724055) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724055) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.