Human DPP4 / CD26 Recombinant Rabbit Monoclonal Antibody [PSH19-28]
cat.: HA724060
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH19-28 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DPP4 aa 29-766 (HA211034). |
| Positive control: | Recombinant Human DPP4 / CD26 protein (HA211034). |
| Subcellular location: | Secreted, Cell membrane, Apical cell membrane, invadopodium membrane, lamellipodium membrane, Cell junction, Membrane raft. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-27] to Human DPP4 / CD26 antibody (Detector) (HA724059) and Recombinant Human DPP4 / CD26 protein (HA211034) as the standard. The reference range value is 39.1-10,000 pg/mL. |
| Uniprot #: | SwissProt: P27487 Human |
| Alternative names: | CD26 antigen ADA-binding protein ADABP ADCP 2 ADCP-2 ADCP2 Adenosine deaminase complexing protein 2 CD 26 CD26 CD26 antigen 3 Dipeptidyl peptidase 4 Dipeptidyl peptidase 4 soluble form Dipeptidyl peptidase IV Dipeptidyl peptidase IV membrane form Dipeptidyl peptidase IV soluble form Dipeptidyl peptidase, intestinal Dipeptidylpeptidase 4 Dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2) Dipeptidylpeptidase IV DPP 4 DPP IV DPP IV estoenzyme DPP4 DPP4_HUMAN DPPIV Intestinal dipeptidyl peptidase T cell activation antigen CD26 T-cell activation antigen CD26 TP 103 TP103 |
Images
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Fig1:
Sandwich ELISA analysis of Human DPP4 matched pair antibodies Capture: HA724060, Human DPP4 / CD26 Rabbit mAb [PSH19-28] Detector: HA724059, Human DPP4 / CD26 Rabbit mAb [PSH19-27] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724060) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human DPP4 / CD26 protein (HA211034) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724059, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native DPP4 in HepG2,MCF-7 culture supernatant and Human urine. Capture: HA724060, Human DPP4 / CD26 Rabbit mAb [PSH19-28] Detector: HA724059, Human DPP4 / CD26 Rabbit mAb [PSH19-27] Interpolated concentration of native DPP4 was measured in duplicate at different sample concentrations and interpolated from the DPP4 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean DPP4 concentration was determined to be 25,703 pg/ml in Human urine,5,564 pg/ml in HepG2 cell culture supernatant and undetectable in MCF-7 cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked DPP4 in cell culture media samples. Capture: HA724060, Human DPP4 / CD26 Rabbit mAb [PSH19-28] Detector: HA724059, Human DPP4 / CD26 Rabbit mAb [PSH19-27] The concentrations of DPP4 were measured in duplicates, interpolated from the DPP4 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.