Human Granzyme K Recombinant Rabbit Monoclonal Antibody [PSH19-30]
cat.: HA724062
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH19-30 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Granzyme K aa 27-264. |
| Positive control: | Recombinant Human Granzyme K protein. |
| Subcellular location: | Secreted, Cytoplasmic granule. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-29] to Human Granzyme K antibody (Capture) (HA724061) and Recombinant Human Granzyme K protein as the standard. The reference range value is 15.6-4,000 pg/mL. |
| Uniprot #: | SwissProt: P49863 Human |
| Alternative names: | Fragmentin 3 Fragmentin-3 Fragmentin3 GRAK_HUMAN Granzyme 3 granzyme K (granzyme 3; tryptase II) granzyme K (serine protease, granzyme 3; tryptase II) Granzyme K Granzyme K precursor Granzyme-3 Granzyme3 GranzymeK GZMK NK TRYP 2 NK TRYP2 NK tryptase 2 NK-TRYP-2 NK-tryptase-2 NKTRYP2 Serine protease granzyme 3 TRYP 2 TRYP2 Tryptase II TryptaseII |
Images
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Fig1:
Sandwich ELISA analysis of Human Granzyme K matched pair antibodies Capture: HA724061, Human Granzyme K Rabbit mAb [PSH19-29] Detector: HA724062, Human Granzyme K Rabbit mAb [PSH19-30] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724061) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Granzyme K protein starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA724062, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Granzyme K in human samples. Capture: HA724061, Human Granzyme K Rabbit mAb [PSH19-29] Detector: HA724062, Human Granzyme K Rabbit mAb [PSH19-30] Interpolated concentration of native Granzyme K was measured in duplicate at different sample concentrations and interpolated from the Granzyme K standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Granzyme K concentration was determined to be 28,512 pg/mL in NK-92 cell supernatant. There was no detectable signal in SH-SY5Y cell supernatant. |
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Fig3:
Interpolated concentrations of native Granzyme K in NK-92 cell extract samples based on a 1000 µg/ml extract load. Capture: HA724061, Human Granzyme K Rabbit mAb [PSH19-29] Detector: HA724062, Human Granzyme K Rabbit mAb [PSH19-30] Interpolated concentration of native Granzyme K was measured in duplicate at different sample concentrations and interpolated from the Granzyme K standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Granzyme K concentration was determined to be 28,512 pg/mL in NK-92 cell extract. |
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Fig4:
Interpolated concentrations of spiked Granzyme K in cell culture media samples. Capture: HA724061, Human Granzyme K Rabbit mAb [PSH19-29] Detector: HA724062, Human Granzyme K Rabbit mAb [PSH19-30] The concentrations of Granzyme K were measured in duplicates, interpolated from the Granzyme K standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.