Phospho-MLKL (S358) Recombinant Rabbit Monoclonal Antibody [PSH19-31]
cat.: HA724063
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH19-31 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser358 of Human MLKL. |
| Positive control: | HT-29 treated with 20μM Z-VAD for 7.5 hours add 20ng/mL hTNF alpha and 100nM SM-164 for last 7 hours cell lysate. |
| Subcellular location: | Cytoplasm, Cell membrane, Nucleus. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q8NB16 Human |
| Alternative names: | 9130019I15Rik FLJ34389 hMLKL Mixed lineage kinase domain like Mixed lineage kinase domain like protein Mixed lineage kinase domain-like protein Mlkl MLKL_HUMAN |
Images
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Fig1:
Western blot analysis of Phospho-MLKL (S358) on different lysates with Rabbit anti-Phospho-MLKL (S358) antibody (HA724063) at 1/5,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 treated with 20μM Z-VAD for 7.5 hours add 20ng/mL hTNF alpha and 100nM SM-164 for last 7 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724063) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.