MuERVL-Gag Recombinant Rabbit Monoclonal Antibody [PSH19-35]
cat.: HA724067
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH19-35 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Gag aa 201-581. |
| Positive control: | ES-D3 cell lysate, ES-W4 cell lysate. |
| Recommended Dilutions:
WB IF-Cell IP |
1:5,000 1:100 1-2μg/sample |
| Uniprot #: | SwissProt: V9H130 Mouse |
Images
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Fig1:
Western blot analysis of MuERVL-Gag on different lysates with Rabbit anti-MuERVL-Gag antibody (HA724067) at 1/5,000 dilution. Lane 1: ES-D3 cell lysate Lane 2: ES-W4 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724067) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
MuERVL-Gag was immunoprecipitated from 0.2 mg ES-W4 cell lysate with HA724067 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724067 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: ES-W4 cell lysate (input) Lane 2: HA724067 IP in ES-W4 cell lysate Lane 3: Rabbit IgG instead of HA724067 in ES-W4 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 2 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.