Cathepsin C Recombinant Rabbit Monoclonal Antibody [PSH19-36]
cat.: HA724068
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH19-36 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Cathepsin C aa 1-463. |
| Positive control: | HeLa cell lysate, U-937 cell lysate, HepG2 cell lysate, SW480 cell lysate, HT-29 cell lysate, Mouse liver tissue lysate, Rat lung tissue lysate, human kidney tissue, human lung tissue, rat kidney tissue, rat lung tissue. |
| Subcellular location: | Lysosome. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:1,000-1:5,000 |
| Uniprot #: | SwissProt: P53634 Human | P97821 Mouse | P80067 Rat |
| Alternative names: | AI047818 CATC CATC_HUMAN Cathepsin C Cathepsin J CPPI CTSC Dipeptidyl peptidase 1 Dipeptidyl peptidase 1 light chain Dipeptidyl peptidase I Dipeptidyl peptidase I exclusion domain chain Dipeptidyl peptidase I heavy chain Dipeptidyl peptidase I light chain Dipeptidyl transferase DPP I DPP-I DPPI EC 3.4.14.1 HMS JP JPD MGC126959 PALS PDON1 PLS |
Images
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Fig1:
Western blot analysis of Cathepsin C on different lysates with Rabbit anti-Cathepsin C antibody (HA724068) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: U-937 cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: SW480 cell lysate (20 µg/Lane) Lane 5: HT-29 cell lysate (20 µg/Lane) Lane 6: Mouse liver tissue lysate (40 µg/Lane) Lane 7: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 52 kDa Observed band size: 55/20 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724068) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cathepsin C antibody (HA724068) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724068) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Cathepsin C antibody (HA724068) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724068) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Cathepsin C antibody (HA724068) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724068) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Cathepsin C antibody (HA724068) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724068) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.