TLR3 Recombinant Rabbit Monoclonal Antibody [PSH19-38]
cat.: HA724070
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH19-38 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 104 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TLR3 aa 24-704. |
| Positive control: | HCT 116 cell lysate, HCT 116 transfected with 50μg/mL Poly(I:C) overnight cell lysate, 293T cell lysate, HT-29 cell lysate, J774A.1 cell lysate. |
| Subcellular location: | Endoplasmic reticulum membrane, Endosome membrane, Early endosome. |
| Recommended Dilutions:
WB |
1:2,000-1:5,000 |
| Uniprot #: | SwissProt: O15455 Human | Q99MB1 Mouse |
| Alternative names: | CD283 CD283 antigen IIAE2 TLR 3 Tlr3 TLR3_HUMAN Toll Like Receptor 3 Toll-like receptor 3 |
Images
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Fig1:
Western blot analysis of TLR3 on different lysates with Rabbit anti-TLR3 antibody (HA724070) at 1/5,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HCT 116 transfected with 50μg/mL Poly(I:C) overnight cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 104 kDa Observed band size: 130/60 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724070) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of TLR3 on different lysates with Rabbit anti-TLR3 antibody (HA724070) at 1/2,000 dilution. Lane 1: 293T cell lysate (negative) (20 µg/Lane) Lane 2: HT-29 cell lysate (no heat) (20 µg/Lane) Lane 3: HT-29 cell lysate treated with deglycosylation (no heat) (20 µg/Lane) Lane 4: J774A.1 cell lysate (30 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 104 kDa Observed band size: 130/60 kDa Exposure time: 1 minute 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724070) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.