ZBTB16 Recombinant Rabbit Monoclonal Antibody [PSH19-39]
cat.: HA724071
| Product Type: | Recombinant Rabbit monoclonal, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH19-39 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 74 kDa |
| Immunogen: | Recombinant protein within human ZBTB16 aa 81-430. |
| Positive control: | LNCaP cell lysate, KG-1 cell lysate, HeLa cell lysate, 293T cell lysate, PC-12 cell lysate, mouse testis tissue, rat testis tissue, E9.5 mouse embryo tissue. |
| Subcellular location: | Nucleus, nuclear body. |
| Recommended Dilutions:
WB IHC-P IP |
1:2,000 1:500-1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q05516 Human |
| Alternative names: | AI467657 lu PLZF Promyelocytic leukemia zinc finger protein ZBT16_HUMAN ZBTB16 Zfp145 Zinc finger and BTB domain containing 16 zinc finger and BTB domain containing protein 16 Zinc finger and BTB domain-containing protein 16 Zinc finger protein 145 (Kruppel like expressed in promyelocytic leukemia) Zinc finger protein 145 Zinc finger protein PLZF Znf145 |
Images
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Fig1:
Western blot analysis of ZBTB16 on different lysates with Rabbit anti-ZBTB16 antibody (HA724071) at 1/2,000 dilution. Lane 1: LNCaP cell lysate (low expression) (20 µg/Lane) Lane 2: KG-1 cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (30 µg/Lane) Lane 4: 293T cell lysate (30 µg/Lane) Lane 5: PC-12 cell lysate (30 µg/Lane) Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724071) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-ZBTB16 antibody (HA724071) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724071) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-ZBTB16 antibody (HA724071) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724071) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded E9.5 mouse embryo tissue with Rabbit anti-ZBTB16 antibody (HA724071) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724071) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded E14.5 mouse embryo tissue (negative) with Rabbit anti-ZBTB16 antibody (HA724071) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724071) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
ZBTB16 was immunoprecipitated from 0.2 mg LNCaP cell lysate with HA724071 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724071 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: LNCaP cell lysate (input) Lane 2: HA724071 IP in LNCaP cell lysate Lane 3: Rabbit IgG instead of HA724071 in LNCaP cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 46 seconds; ECL: K1801 |
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