TNFRSF10A Recombinant Rabbit Monoclonal Antibody [PSH19-41]
cat.: HA724073
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH19-41 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TNFRSF10A aa 263-468. |
| Positive control: | HCT 116 cell lysate, HeLa cell lysate, K-562 cell lysate, U266 cell lysate, MCF7 cell lysate, HCT 116. |
| Subcellular location: | Cell membrane, Membrane raft, Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: O00220 Human |
| Alternative names: | Apo2 CD261 Cytotoxic TRAIL receptor Death receptor 4 DR4 NF related apoptosis-inducing ligand receptor 1 TNF receptor superfamily member 10a TNF-related apoptosis-inducing ligand receptor 1 TNFRSF10A TR10A_HUMAN TRAIL receptor 1 TRAIL-R1 TRAILR 1 TRAILR1 Tumor necrosis factor receptor superfamily member 10A Tumor necrosis factor receptor superfamily member 10a variant 2 Tumor necrosis factor receptor superfamily member 10a |
Images
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Fig1:
Western blot analysis of TNFRSF10A on different lysates with Rabbit anti-TNFRSF10A antibody (HA724073) at 1/5,000 dilution. Lane 1: HCT 116 cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (negative) (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: U266 cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Predicted band size: 50 kDa Observed band size: 40-55 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724073) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HCT 116 (positive) and 293T (negative) labeling TNFRSF10A with Rabbit anti-TNFRSF10A antibody (HA724073) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TNFRSF10A antibody (HA724073) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of 293T (left, negative) and HCT 116 (right, positive) cells labeling TNFRSF10A. Cells were fixed and permeabilized. Then stained with the primary antibody (HA724073, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
TNFRSF10A was immunoprecipitated from 0.2 mg HCT 116 cell lysate with HA724073 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724073 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HCT 116 cell lysate (input) Lane 2: HA724073 IP in HCT 116 cell lysate Lane 3: Rabbit IgG instead of HA724073 in HCT 116 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 46 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.