NCR1 Recombinant Rabbit Monoclonal Antibody [PSH19-42]
cat.: HA724074
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, FC, WB |
| Clonality: | Monoclonal |
| Clone number: | PSH19-42 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse NCR1 aa 86-255. |
| Positive control: | Mouse spleen tissue, rat spleen tissue, C57BL.6 mouse spleen cells. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IHC-Fr IHC-P FC WB |
1:500 1:1,000 1:1,000 1:5,000 |
| Uniprot #: | SwissProt: Q8C567 Mouse | Q9Z0H5 Rat |
| Alternative names: | Activating NK receptor NK p46 CD335 FLJ99094 hNKp46 Ly94 Lymphocyte antigen 94 Lymphocyte antigen 94 homolog (activating NK receptor; NK p46) Lymphocyte antigen 94 homolog Natural cytotoxicity triggering receptor 1 Natural killer cell p46-related protein NCR1 NCT1 NCTR1_HUMAN NK cell activating receptor NK cell-activating receptor NK-p46 NKp46 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: spleen Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-NCR1 antibody (HA724074) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724074) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-NCR1 antibody (HA724074) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724074) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of C57BL.6 mouse spleen cells labeling NCR1 (HA724074) and NK1.1-FITC. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA724074, 1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 647 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1123) at 1/1,000 dilution for 30 minutes at +4℃. |
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Fig5:
Western blot analysis of NCR1 on NCR1 recombinant protein with Rabbit anti-NCR1 antibody (HA724074) at 1/5,000 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724074) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.