RIP Recombinant Rabbit Monoclonal Antibody [PSH19-57]
cat.: HA724083
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH19-57 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 76 kDa |
| Isotype: | IgG |
| Positive control: | HT-29 cell lysate, HeLa cell lysate, Raji cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, MEF cell lysate, C6 cell lysate. |
| Subcellular location: | Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q13546 Human | Q60855 Mouse Entrez Gene: 306886 Rat |
| Alternative names: | Cell death protein RIP FLJ39204 OTTHUMP00000039163 Receptor (TNFRSF) interacting serine threonine kinase 1 receptor interacting protein 1 Receptor interacting protein Receptor interacting protein kinase 1 Receptor interacting serine threonine protein kinase 1 Receptor TNFRSF interacting serine threonine kinase 1 Receptor-interacting protein 1 Receptor-interacting serine/threonine-protein kinase 1 Rinp RIP 1 RIP Rip-1 RIP1 RIPK 1 Ripk1 RIPK1_HUMAN Serine threonine protein kinase RIP Serine/threonine-protein kinase RIP |
Images
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Fig1:
Western blot analysis of RIP on different lysates with Rabbit anti-RIP antibody (HA724083) at 1/5,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HeLa cell lysate Lane 3: Raji cell lysate Lane 4: COS-1 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: MEF cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 76 kDa Observed band size: 75 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724083) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.