IL-10RB Recombinant Rabbit Monoclonal Antibody [PSH19-60]
cat.: HA724086
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH19-60 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IL-10RB aa 1-220. |
| Positive control: | THP-1 cell lysate, HDLM-2 cell lysate, HUVEC cell lysate, THP-1. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000 1:500 1:1,000 |
| Uniprot #: | SwissProt: Q08334 Human |
| Alternative names: | Cd210 CDw210b CRF2 4 CRF2-4 CRFB4 Cytokine receptor class II Cytokine receptor class II CRF2 4 Cytokine receptor class-II member 4 Cytokine receptor family 2 member 4 Cytokine receptor family II member 4 D21S58 D21S66 I10R2_HUMAN IL-10 receptor subunit beta IL-10R subunit 2 IL-10R subunit beta IL-10R2 IL-10RB IL10R2 IL10RB Interleukin 10 receptor beta Interleukin-10 receptor subunit 2 Interleukin-10 receptor subunit beta |
Images
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Fig1:
Western blot analysis of IL-10RB on different lysates with Rabbit anti-IL-10RB antibody (HA724086) at 1/5,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 cell lysate (no heat) Lane 3: HDLM-2 cell lysate Lane 4: HUVEC cell lysate Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 55-75 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724086) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of IL-10RB on different lysates with Rabbit anti-IL-10RB antibody (HA724086) at 1/5,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 35-75 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724086) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of THP-1 cells labeling IL-10RB with Rabbit anti-IL-10RB antibody (HA724086) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-10RB antibody (HA724086) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Flow cytometric analysis of THP-1 cells labeling IL-10RB. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA724086, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.