Biotin Conjugated Human CCL18 Recombinant Rabbit Monoclonal Antibody [PSH19-65]
cat.: HA724089B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH19-65 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CCL18 aa 21-89 (HA211080). |
| Positive control: | Recombinant Human CCL18 protein (HA211080). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-63] to Human CCL18 antibody (Capture) (HA724087) and Recombinant CCL18 protein (HA211080) as the standard. The reference range value is 3.9-1,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P55774 Human |
| Alternative names: | Alternative macrophage activation associated CC chemokine 1 Alternative macrophage activation-associated CC chemokine 1 AMAC-1 AMAC1 CC chemokine PARC CCL18 CCL18(4-69) CCL18_HUMAN CKb7 DC-CK1 DCCK1 DCCK1 Dendritic cell chemokine 1 Macrophage inflammatory protein 4 MIP-4 MIP4 PARC Pulmonary and activation regulated chemokine Pulmonary and activation-regulated chemokine SCYA18 Small inducible cytokine A18 Small inducible cytokine A18 Small-inducible cytokine A18 |
Images
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Fig1:
Sandwich ELISA analysis of Human CCL18 matched pair antibodies Capture: HA724087, Human CCL18 Rabbit mAb [PSH19-63] Detector: HA724089, Human CCL18 Rabbit mAb [PSH19-65] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724087) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL18 protein (HA211080) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA724089, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CCL18 in human serum and HeLa cell culture supernatant. Capture: HA724087, Human CCL18 Rabbit mAb [PSH19-63] Detector: HA724089, Human CCL18 Rabbit mAb [PSH19-65] The concentrations of CCL18 were measured in duplicates, interpolated from the CCL18 standard curve and corrected for sample dilution. Undiluted samples are human serum 4% and HeLa cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL18 concentration was determined to be 33.1 ng/ml in human serum and undetectable in HeLa cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked CCL18 in human cell culture media samples. Capture: HA724087, Human CCL18 Rabbit mAb [PSH19-63] Detector: HA724089, Human CCL18 Rabbit mAb [PSH19-65] The concentrations of CCL18 were measured in duplicates, interpolated from the CCL18 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.