Human Complement factor D Recombinant Rabbit Monoclonal Antibody [PSH19-67]
cat.: HA724091
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH19-67 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CFD aa 26-253 (HA211128). |
| Positive control: | Recombinant Human Complement factor D protein (HA211128). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-66] to Human Complement factor D antibody (Capture) (HA724090) and Recombinant Human Complement factor D protein (HA211128) as the standard. The reference range value is 39.1-5,000 pg/mL. |
| Uniprot #: | SwissProt: P00746 Human |
| Alternative names: | Adipsin Adipsin/complement factor D Adn C3 convertase activator CFAD_HUMAN CFD Complement factor D complement factor D preproprotein D component of complement DF FactorD PFD Properdin factor D |
Images
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Fig1:
Sandwich ELISA analysis of human Complement Factor D matched pair antibodies Capture: HA724090, Human Complement factor D Rabbit mAb [PSH19-66] Detector: HA724091, Human Complement factor D Rabbit mAb [PSH19-67] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724090) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Complement factor D protein (HA211128) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA724091, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Complement Factor D in U-937 and HepG2 cell culture supernatant. Capture: HA724090, Human Complement factor D Rabbit mAb [PSH19-66] Detector: HA724091, Human Complement factor D Rabbit mAb [PSH19-67] The concentrations of Complement Factor D were measured in duplicates, interpolated from the Complement Factor D standard curve and corrected for sample dilution. Undiluted samples are U-937 cell culture supernatant 25% and HepG2 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor D concentration was determined to be 10,911 pg/ml in U-937 cell culture supernatant and undetectable in HepG2 cell culture supernatant. |
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Fig3:
Interpolated concentrations of native Complement Factor D in human serum samples. Capture: HA724090, Human Complement factor D Rabbit mAb [PSH19-66] Detector: HA724091, Human Complement factor D Rabbit mAb [PSH19-67] The concentrations of Complement Factor D were measured in duplicates, interpolated from the Complement Factor D standard curve and corrected for sample dilution. Undiluted samples are human serum 0.1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor D concentration was determined to be 1,242 ng/ml in human serum. |
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Fig4:
Interpolated concentrations of spiked Complement Factor D in human cell culture media samples. Capture: HA724090, Human Complement factor D Rabbit mAb [PSH19-66] Detector: HA724091, Human Complement factor D Rabbit mAb [PSH19-67] The concentrations of Complement Factor D were measured in duplicates, interpolated from the Complement Factor D standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.