HRP Conjugated Human Complement factor D Recombinant Rabbit Monoclonal Antibody [PSH19-67]
cat.: HA724091H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH19-67 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CFD aa 26-253 (HA211128). |
| Positive control: | Recombinant Human Complement factor D protein (HA211128). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-66] to Human Complement factor D antibody (Capture) (HA724090) and Recombinant Human Complement factor D protein (HA211128) as the standard. The reference range value is 39.1-5,000 pg/mL. |
| Uniprot #: | SwissProt: P00746 Human |
| Alternative names: | Adipsin Adipsin/complement factor D Adn C3 convertase activator CFAD_HUMAN CFD Complement factor D complement factor D preproprotein D component of complement DF FactorD PFD Properdin factor D |
Images
|
Fig1:
Sandwich ELISA analysis of human Complement Factor D matched pair antibodies Capture: HA724090, Human Complement factor D Rabbit mAb [PSH19-66] Detector: HA724091, Human Complement factor D Rabbit mAb [PSH19-67] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724090) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Complement factor D protein (HA211128) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA724091, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.