Myelin Protein Zero Recombinant Rabbit Monoclonal Antibody [PSH19-69]
cat.: HA724093
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P, IP, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH19-69 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Positive control: | Mouse skeletal muscle tissue lysate, Mouse skin tissue lysate, Rat spinal cord tissue lysate, Rat skeletal muscle tissue lysate, Rat skin tissue lysate, Mouse spinal cord tissue lysate, mouse dorsal root ganglion tissue, rat dorsal root ganglion tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-Fr IHC-P IP IF-Tissue |
1:2,000 1:500 1:1,000 1-2μg/sample 1:500 |
| Uniprot #: | SwissProt: P27573 Mouse | P06907 Rat |
| Alternative names: | Charcot Marie Tooth neuropathy 1B CHM CMT1 CMT1B CMT2I CMT2J CMT4E CMTDI3 CMTDID DSS HMSNIB MPP MPZ Myelin peripheral protein Myelin protein P0 Myelin protein zero MYP0_HUMAN P0 |
Images
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Fig1:
Western blot analysis of Myelin Protein Zero on different lysates with Rabbit anti-Myelin Protein Zero antibody (HA724093) at 1/2,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate (20 µg/Lane) Lane 2: Mouse skin tissue lysate (20 µg/Lane) Lane 3: Rat spinal cord tissue lysate (10 µg/Lane) Lane 4: Rat skeletal muscle tissue lysate (10 µg/Lane) Lane 5: Rat skin tissue lysate (10 µg/Lane) Predicted band size: 28 kDa Observed band size: 25 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724093) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Myelin Protein Zero on different lysates with Rabbit anti-Myelin Protein Zero antibody (HA724093) at 1/2,000 dilution. Lane 1: Mouse spinal cord tissue lysate Lane 2: Mouse liver tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 28 kDa Observed band size: 25 kDa Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724093) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Application: IHC-Fr Species: Mouse Site: spinal cord Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse dorsal root ganglion tissue with Rabbit anti-Myelin Protein Zero antibody (HA724093) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724093) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue with Rabbit anti-Myelin Protein Zero antibody (HA724093) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724093) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Myelin Protein Zero was immunoprecipitated from 0.2 mg Mouse spinal cord tissue lysate with HA724093 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724093 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse spinal cord tissue lysate (input) Lane 2: HA724093 IP in Mouse spinal cord tissue lysate Lane 3: Rabbit IgG instead of HA724093 in Mouse spinal cord tissue lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 5 seconds; ECL: K1801 |
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Fig7:
Application: IF-Tissue Species: Mouse Site: dorsal root ganglion Sample: Paraffin-embedded section Antibody concentration: 1/500 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.