UCP2 Recombinant Rabbit Monoclonal Antibody [PSH19-75]
cat.: HA724099
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH19-75 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human UCP2 aa 181-230. |
| Positive control: | HeLa cell lysate, Huh7 cell lysate, HT-29 cell lysate, C6 cell lysate, COS-1 cell lysate, Mouse liver tissue lysate, THP-1 cell lysate, THP-1 treated with 1μg/mL LPS for 24 hours cell lysate, THP-1 treated with 100ng/mL LPS for 48 hours cell lysate, RAW264.7 cell lysate, RAW264.7 treated with 1μg/mL LPS for 24 hours cell lysate, RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P55851 Human | P70406 Mouse | P56500 Rat |
| Alternative names: | BMIQ4 Mitochondrial uncoupling protein 2 SLC25A8 Solute carrier family 25 member 8 UCP 2 ucp2 UCP2_HUMAN UCPH Uncoupling protein 2 Uncoupling protein 2 mitochondrial proton carrier |
Images
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Fig1:
Western blot analysis of UCP2 on different lysates with Rabbit anti-UCP2 antibody (HA724099) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Huh7 cell lysate (20 µg/Lane) Lane 3: HT-29 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: COS-1 cell lysate (20 µg/Lane) Lane 6: Mouse liver tissue lysate (20 µg/Lane) Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724099) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of UCP2 on different lysates with Rabbit anti-UCP2 antibody (HA724099) at 1/5,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: THP-1 treated with 1μg/mL LPS for 24 hours cell lysate (20 µg/Lane) Lane 3: THP-1 treated with 100ng/mL LPS for 48 hours cell lysate (20 µg/Lane) Lane 4: RAW264.7 cell lysate (20 µg/Lane) Lane 5: RAW264.7 treated with 1μg/mL LPS for 24 hours cell lysate (20 µg/Lane) Lane 6: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate (20 µg/Lane) Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724099) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.