Mouse GP130 / IL6ST Recombinant Rabbit Monoclonal Antibody [PSH19-82]
cat.: HA724106
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH19-82 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse GP130 / IL6ST aa 23-617 (HA211196). |
| Positive control: | Recombinant Mouse GP130 / IL6ST protein (HA211196). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-83] to Mouse GP130 / IL6ST antibody (Detector) (HA724107) and Recombinant Mouse GP130 / IL6ST protein (HA211196) as the standard. The reference range value is 39.1-10,000 pg/mL. |
| Uniprot #: | SwissProt: Q00560 Mouse |
| Alternative names: | CD130 CD130 antigen CDw130 gp130 GP130 RAPS IL 6R beta IL-6 receptor subunit beta IL-6R subunit beta IL-6R-beta IL-6RB IL6 ST IL6RB_HUMAN IL6ST Interleukin 6 receptor subunit beta Interleukin receptor beta chain Interleukin-6 receptor subunit beta Interleukin-6 signal transducer Membrane glycoprotein 130 Membrane glycoprotein gp130 Oncostatin M receptor Oncostatin M receptor alpha subunit Oncostatin-M receptor subunit alpha |
Images
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Fig1:
Sandwich ELISA analysis of Mouse GP130 / IL6ST matched pair antibodies Capture: HA724106, Mouse GP130 / IL6ST Rabbit mAb [PSH19-82] Detector: HA724107, Mouse GP130 / IL6ST Rabbit mAb [PSH19-83] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724106) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse GP130 / IL6ST protein (HA211196) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA724107, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native GP130 in Mouse serum. Capture: HA724106, Mouse GP130 / IL6ST Rabbit mAb [PSH19-82] Detector: HA724107, Mouse GP130 / IL6ST Rabbit mAb [PSH19-83] Interpolated concentration of native GP130 was measured in duplicate at different sample concentrations and interpolated from the GP130 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean GP130 concentration was determined to be 20,186 pg/ml in Mouse serum. |
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Fig3:
Interpolated concentrations of native GP130 in NIH/3T3 Cell extract samples based on a 1000 µg/ml extract load. Capture: HA724106, Mouse GP130 / IL6ST Rabbit mAb [PSH19-82] Detector: HA724107, Mouse GP130 / IL6ST Rabbit mAb [PSH19-83] Interpolated concentration of native GP130 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean GP130 concentration was determined to be 25,864 pg/ml in NIH/3T3 Cell extract. |
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Fig4:
Interpolated concentrations of spiked GP130 in cell culture media samples. Capture: HA724106, Mouse GP130 / IL6ST Rabbit mAb [PSH19-82] Detector: HA724107, Mouse GP130 / IL6ST Rabbit mAb [PSH19-83] The concentrations of GP130 were measured in duplicates, interpolated from the GP130 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.