Rat CCL2 / MCP-1 Recombinant Rabbit Monoclonal Antibody [PSH19-84]
cat.: HA724108
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH19-84 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Rat CCL2 / MCP-1 aa 24-148 (HA211114). |
| Positive control: | Recombinant Rat CCL2 / MCP-1 protein (HA211114). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-85] to Rat CCL2 / MCP-1 antibody (Detector) (HA724109) and Recombinant Rat CCL2 / MCP-1 protein (HA211114) as the standard. The reference range value is 15.6-2,000 pg/mL. |
| Uniprot #: | SwissProt: P14844 Rat |
| Alternative names: | C-C motif chemokine 2 CCL2 CCL2_HUMAN Chemokine (C C motif) ligand 2 GDCF 2 GDCF-2 GDCF2 HC11 HSMCR30 JE MCAF MCP 1 MCP-1 MCP1 MGC9434 Monocyte chemoattractant protein 1 Monocyte chemotactic and activating factor Monocyte chemotactic protein 1 Monocyte chemotactic protein-1 Monocyte secretory protein JE SCYA2 Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) Small inducible cytokine A2 Small inducible cytokine subfamily A (Cys Cys), member 2 Small-inducible cytokine A2 SMC CF SMC-CF SMCCF |
Images
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Fig1:
Sandwich ELISA analysis of Rat CCL2 matched pair antibodies Capture: HA724108, Rat CCL2 / MCP-1 Rabbit mAb [PSH19-84] Detector: HA724109, Rat CCL2 / MCP-1 Rabbit mAb [PSH19-85] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724108) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat CCL2 / MCP-1 protein (HA211114) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA724109, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CCL2 in rat serum samples. Capture: HA724108, Rat CCL2 / MCP-1 Rabbit mAb [PSH19-84] Detector: HA724109, Rat CCL2 / MCP-1 Rabbit mAb [PSH19-85] The concentrations of CCL2 were measured in duplicates, interpolated from the CCL2 standard curve and corrected for sample dilution. Undiluted samples are rat serum 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL2 concentration was determined to be 3,430 pg/ml in rat serum. |
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Fig3:
Interpolated concentrations of spiked CCL2 in rat cell culture media samples. Capture: HA724108, Rat CCL2 / MCP-1 Rabbit mAb [PSH19-84] Detector: HA724109, Rat CCL2 / MCP-1 Rabbit mAb [PSH19-85] The concentrations of CCL2 were measured in duplicates, interpolated from the CCL2 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media without FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.