Mouse NCR1 / NKp46 Recombinant Rabbit Monoclonal Antibody [PSH19-89]
cat.: HA724113
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH19-89 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse NCR1 / NKp46 aa 119-255. |
| Positive control: | Recombinant Mouse NCR1 / NKp46 protein. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-88] to Mouse NCR1 / NKp46 antibody (Capture) (HA724112) and Recombinant Mouse NCR1 / NKp46 protein as the standard. The reference range value is 39.1-5,000 pg/mL. |
| Uniprot #: | SwissProt: Q8C567 Mouse |
| Alternative names: | Activating NK receptor NK p46 CD335 FLJ99094 hNKp46 Ly94 Lymphocyte antigen 94 Lymphocyte antigen 94 homolog (activating NK receptor; NK p46) Lymphocyte antigen 94 homolog Natural cytotoxicity triggering receptor 1 Natural killer cell p46-related protein NCR1 NCT1 NCTR1_HUMAN NK cell activating receptor NK cell-activating receptor NK-p46 NKp46 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse NCR1 matched pair antibodies Capture: HA724112, Mouse NCR1 / NKp46 Rabbit mAb [PSH19-88] Detector: HA724113, Mouse NCR1 / NKp46 Rabbit mAb [PSH19-89] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724112) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse NCR1 / NKp46 protein starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA724113, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of spiked NCR1 in cell culture media samples. Capture: HA724112, Mouse NCR1 / NKp46 Rabbit mAb [PSH19-88] Detector: HA724113, Mouse NCR1 / NKp46 Rabbit mAb [PSH19-89] The concentrations of NCR1 were measured in duplicates, interpolated from the NCR1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.