Wnt3a Recombinant Rabbit Monoclonal Antibody [PSH19-96]
cat.: HA724116
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH19-96 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Positive control: | HCT 116 cell lysates, mouse E14.5 embryo tissue. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:200 1-2μg/sample |
| Uniprot #: | SwissProt: P56704 Human | P27467 Mouse |
| Alternative names: | Protein Wnt 3a Precursor Protein Wnt-3a Wingless type MMTV integration site family member 3A Wnt 3a wnt3a WNT3A protein WNT3A_HUMAN |
Images
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Fig1:
Western blot analysis of Wnt3a on HCT 116 cell lysates with Rabbit anti-Wnt3a antibody (HA724116) at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 39 kDa Observed band size: 39 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724116) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue with Rabbit anti-Wnt3a antibody (HA724116) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724116) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Wnt3a was immunoprecipitated from 0.2 mg HCT 116 cell lysate with HA724116 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724116 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HCT 116 cell lysate (input) Lane 2: HA724116 IP in HCT 116 cell lysate Lane 3: Rabbit IgG instead of HA724116 in HCT 116 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 minutes; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.