HRP Conjugated Human Apolipoprotein E Recombinant Rabbit Monoclonal Antibody [PSH20-19]
cat.: HA724130H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH20-19 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Apolipoprotein E aa 19-317 (HA211078). |
| Positive control: | Recombinant Human Apolipoprotein E protein (HA211078). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix, Extracellular vesicle, Endosome, multivesicular body. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-18] to Human Apolipoprotein E antibody (Capture) (HA724129) or Rabbit monoclonal [PSH20-20] to Human Apolipoprotein E antibody (Capture) (HA724131) and Recombinant Human Apolipoprotein E protein (HA211078) as the standard. The reference range value is 781.25-100,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P02649 Human |
| Alternative names: | AD2 Apo-E APOE APOE_HUMAN APOEA Apolipoprotein E Apolipoprotein E3 ApolipoproteinE Apoprotein LDLCQ5 LPG |
Images
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Fig1:
Sandwich ELISA analysis of Human Apolipoprotein E matched pair antibodies Capture: HA724129, Human Apolipoprotein E Rabbit mAb [PSH20-18] Detector: HA724130, Human Apolipoprotein E Rabbit mAb [PSH20-19] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724129) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted APOE protein starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA724130, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.