Human Plasminogen Recombinant Rabbit Monoclonal Antibody [PSH20-21]
cat.: HA724132
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH20-21 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Plasminogen aa 98-370 (HA211379). |
| Positive control: | Recombinant Human Plasminogen protein (HA211379). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-22] to Human Plasminogen antibody (Detector) (HA724133) and Recombinant Human Plasminogen protein (HA211379) as the standard. The reference range value is 7.8-2,000 pg/mL. |
| Uniprot #: | SwissProt: P00747 Human |
| Alternative names: | Plasmin Plasmin heavy chain A Plasmin light chain B Plasminogen PLG PLMN_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of Human Plasminogen matched pair antibodies Capture: HA724132, Human Plasminogen Rabbit mAb [PSH20-21] Detector: HA724133, Human Plasminogen Rabbit mAb [PSH20-22] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724132) diluted in carbonate/bicarbonate buffer, at a concentHumanion of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.2% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Plasminogen protein (HA211379) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA724133, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB SubstHumane for 10 minutes at room tempeHumanure in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Plasminogen in human serum samples. Capture: HA724132, Human Plasminogen Rabbit mAb [PSH20-21] Detector: HA724133, Human Plasminogen Rabbit mAb [PSH20-22] The concentrations of Plasminogen were measured in duplicates, interpolated from the Plasminogen standard curve and corrected for sample dilution. Undiluted samples are human serum 0.0025%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Plasminogen concentration was determined to be 49.2 µg/ml in human serum. |
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Fig3:
Interpolated concentrations of spiked Plasminogen in human cell culture media samples. Capture: HA724132, Human Plasminogen Rabbit mAb [PSH20-21] Detector: HA724133, Human Plasminogen Rabbit mAb [PSH20-22] The concentrations of Plasminogen were measured in duplicates, interpolated from the Plasminogen standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.